biotinylated universal antibody (horse anti-mouse/rabbit igg) (Vector Laboratories)

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Vector Laboratories biotinylated universal antibody (horse anti-mouse/rabbit igg)
Biotinylated Universal Antibody (Horse Anti Mouse/Rabbit Igg), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horse+anti+mouse/Biotinylated+Universal+Antibody+(Horse+Anti-Mouse%2FRabbit+IgG)/custom%40ba-1400%4042532140
Average 96 stars, based on 7660 article reviews

biotinylated universal antibody (horse anti-mouse/rabbit igg) - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Intestinal interoceptive dysfunction drives age-associated cognitive decline.
Article Snippet: .. Primary antibody was rinsed off with 0.1 M Tris for 5 min, and then incubated with goat anti-rabbit (Vector) or horse anti-mouse (Vector) biotinylated IgG in 0.1 M Tris/2% FBS 1:1000 for 1 h. Biotinylated antibody was rinsed off with 0.1 M Tris for 5 min, then incubated with avidin-biotin solution (Vector) for 1 h. Slides were then rinsed for 5 min with 0.1 M Tris and then developed with ImmPACT DAB peroxidase substrate (Vector) and counterstained briefly with haematoxylin. .. Immunohistochemistry sections were scanned with a 3DHISTECH Laminar Scanner (Perkin Elmer) and quantification was done with QuPath90.

Article Title: Helicobacter pylori infection and α-synuclein pathology drive parallel neurodegenerative pathways in the substantia nigra
Article Snippet: .. For immunohistochemistry, the primary antibody was rinsed off with 0.1 M Tris and incubated in 0.1 M Tris for 5 min, then incubated with goat anti-rabbit (Vector BA1000, RRID: AB_2313606) or horse anti-mouse (Vector BA2000, RRID: AB_2313581) biotinylated IgG in 0.1 M Tris/2% FBS 1:1000 for 1 h. The biotinylated antibody was rinsed off with 0.1 M Tris for 5 min, then incubated with avidin-biotin solution (Vector PK-6100, RRID: AB_2336819) for 1 h. Slides were then rinsed with 0.1 M Tris and washed for 5 min with 0.1 M Tris. .. The slides were then developed with ImmPACT DAB peroxidase substrate (Vector SK-4105, RRID: AB_2336520) and counterstained briefly with Harris Hematoxylin (Fisher 67–650-01).

Article Title: Helicobacter pylori infection and α-synuclein pathology drive parallel neurodegenerative pathways in the substantia nigra.
Article Snippet: .. For immunohistochemistry, the primary antibody was rinsed off with 0.1 M Tris and incubated in 0.1 M Tris for 5 min, then incubated with goat anti-rabbit (Vector BA1000, RRID: AB_2313606) or horse anti-mouse (Vector BA2000, RRID: AB_2313581) biotinylated IgG in 0.1 M Tris/2% FBS 1:1000 for 1 h. The biotinylated antibody was rinsed off with 0.1 M Tris for 5 min, then incubated with avidin-biotin solution (Vector PK-6100, RRID: AB_2336819) for 1 h. Slides were then rinsed with 0.1 M Tris and washed for 5 min with 0.1 M Tris. .. The slides were then developed with ImmPACT DAB peroxidase substrate (Vector SK-4105, RRID: AB_2336520) and counterstained briefly with Harris Hematoxylin (Fisher 67–650-01).

Article Title: Spatial lipidomics identifies α-synuclein-induced lipid changes in an AAV-induced Parkinsonian mouse model.
Article Snippet: Afterwards, they were incubated overnight at 4 ◦C with corresponding primary antibodies: chicken anti-GFP (1:500; ab13970, Abcam), mouse anti-human α-synuclein (1:500; sc12767, Santa Cruz). .. On the second day, after washing in PBS for 4 × 5 min, sections were incubated for 2 h at RT with corresponding biotinylated secondary antibodies: goat antichicken (1:300; SAB3700204, Merck) and horse anti-mouse (1:300; BA2001, Vector Laboratories). .. After washing in PBS for 4 × 5 min, streptavidin-HRP complex (Vectastain Elite ABC kit; Vector Laboratories) was applied to the sections for 1 h at RT.

Article Title: Intestinal interoceptive dysfunction drives age-associated cognitive decline
Article Snippet: .. Primary antibody was rinsed off with 0.1 M Tris for 5 min, and then incubated with goat anti-rabbit (Vector) or horse anti-mouse (Vector) biotinylated IgG in 0.1 M Tris/2% FBS 1:1000 for 1 h. Biotinylated antibody was rinsed off with 0.1 M Tris for 5 min, then incubated with avidin-biotin solution (Vector) for 1 h. Slides were then rinsed for 5 min with 0.1 M Tris and then developed with ImmPACT DAB peroxidase substrate (Vector) and counterstained briefly with haematoxylin. .. Immunohistochemistry sections were scanned with a 3DHISTECH Laminar Scanner (Perkin Elmer) and quantification was done with QuPath .

Avidin-Biotin Assay:

Immunohistochemistry:

other:

Article Title: Hippo signaling differentially regulates distal progenitor subpopulations and their transitional states to construct the mammalian lungs.
Article Snippet: For immunostaining46, 52, 53, sections were dewaxed with xylene (VWR 89370-088) then rehydrated with graded alcohol solutions (100% ethanol, 95% ethanol, 80% ethanol; Decon V1001).

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